Table S5 Verification experiment of orthogonal experiment L9 (3 4) Table S4 Variance analysis of orthogonal experiment L9 (3 4) Table S3 Orthogonal experiment L9 (3 4) of sample extraction and range analysis Table S2 Targets related to the anti-hypertension activity of AVF Table S1 Information of commercial AVF samples Pca317-Supplement materials.docxWord 2007 document The chemical markers obtained from this study could be applicable for efficacy-related quality control of AVF. Hierarchical cluster analysis and heatmap analysis showed that 14 batches of commercial AVF samples could be divided into three main groups. The quantitative data showed that the total contents of seven marker components even showed 2-fold variation among 14 batches of commercial AVF samples with RSD values ranging from 12.15% to 75.61%. Thirteen prototype constituents were unequivocally or tentatively characterized in serum samples, among which quercetin, kaempferol, hyperoside, isoquercitrin, chlorogenic acid, cryptochlorogenic acid, and neochlorogenic acid were identified as dominant chemicals related to anti-hypertensive efficacy. Subsequently, a quantitative method was established using UPLC with diode array detection (DAD) and applied for quality evaluation of commercial AVF samples. Network pharmacology was further used to identify anti-hypertension-related chemical markers. Ultra-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS/MS) was applied to identify the absorbed AVF constituents in rat serum. This study was intended to integrate serum pharmacochemistry and network pharmacology to identify chemical markers of AVF and establish an efficacy-related quality control method of AVF. Chemical markers are crucial for the quality control of herbal medicines however, the therapeutic components of AVF remain to be well elucidated. Apocyni Veneti Folium (AVF) is a commonly used traditional Chinese medicinal herb for the treatment of hypertension.
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